Vascular-tubular relationships in the mammalian kidney
overview - introduction - technique - back to index
Techniques used for staining the kidneys or filling the renal blood vessels
Staining with Alcian Blue
Alcian blue was infused in vivo by intravenous route. Kidneys were then removed and dehydrated in alcoohol. Then, the renal tissue was cleared by impregnation with methylsalycilate.
Vascular filling with Microfil Silicone Rubber®
The renal vasculature has been filled post mortem with white silicone rubber (Microfil Silicone Rubber®) either through arterial or through venous route. Kidneys were then removed and dehydrated in alcoohol. Then, the renal tissue was cleared by impregnation with methylsalycilate.
See more detailed description of these techniques in the following papers.
Bankir L, Hollenberg NK:
In vivo staining of the kidney with Alcian Blue : an adjunct to morphological and physiological studies.
Renal Physiol 6: 151-155, 1983
Bankir L, Kaissling B, Rouffignac-de C, Kriz W:
The vascular organization of the kidney of Psammomys obesus.
Anat Embryol 155: 149-160, 1979
Bankir L, Rouffignac-de C:
Urinary concentrating ability : Insights from comparative anatomy (Editorial review).
Am. J. Physiol. 249 (Regulatory Integr. Comp. Physiol. 18): R643-R666, 1985
